Friday, April 21, 2017

Fossilized Microbial Forms in Baltic and Goan Amber- A Comparative Pioneer Study



Fossilized Microbial Forms  in Baltic and  Goan Amber- A Comparative Pioneer Study
Dabolkar Sujata*  and Kamat Nandkumar
Department of Botany, Goa University , Taleigao, Goa, 403206 , India
Corresponding Author  Email : nandkamat@gmail.com
*Presenting Authors Email : sujatadabolkar@gmail.com
ABSTRACT
This paper is based on surveys, exploration  and standardization of techniques to recover rare amber samples from sands in Goa and identify specimens embeded with distinct microbial fossils based on studies on reference samples of imported Baltic Amber. We developed techniques to locate, detect and identify amber samples in local sands. In this pioneer study we report presumptive microbial forms such as Actinobacteria and fungi in amber samples of Goa throwing light on microbial paleobiodiversity. Baltic amber (Succinate) is  fossilized resin belonging to the Eocene period (44-49 million years old) derived from the Araucariaceae and Leguminoseae family of trees containing up to 8% of Succinic acid and compounds such as terpenoids and phenolic derivatives. Sooty moulds in the amber fossils have been studied (Schmidt et al., 2013). Samples of imported Baltic amber were validated, studied and used as reference for identification and characterization of amber found in rivers sands of Goa. FTIR Spectroscopic tests diagnostic of presence of Succinate proved that both samples meet the criteria as plant derived Succinate containing products. Fossil fungi in Baltic amber were observed, and compared with similar forms in rare amber fragments of Goa. These samples were subjected to butanol treatment and microscopic examination. Actinobacterial and fungal forms embedded in local amber were compared with similar forms found in imported Baltic Amber and those published in literature. Detection of fossilized Actinobacterial and fungal forms has shown us the potential for further studies for comprehensive collection and microscopic examination of such paleomicrobial forms in rare local amber samples.